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KMID : 0354720070310020113
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Journal of Korean Diabetes Association
2007 Volume.31 No. 2 p.113 ~ p.122
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Effects of Islet Transplantation on Endogenous β-cell Regeneration after Partial Pancreatectomy in Rodents
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Jung Hye-Seung
Ahn You-Ran
Oh Seung-Hoon
Kim Tae-Hyun
Jung Jung-Hwa
Hwang You-Chul
Kang Mi-Ra
Bae Yong-Suk
Kim Young-Suk
Chung Jae-Hoon
Min Yong-Ki
Lee Myung-Shik
Lee Moon-Kyu
Kim Kwang-Won
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Abstract
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Background: Islet transplantation is one of regimens supplying the deficient insulin in diabetes patients, but
the effects of islet grafts on the changes of endogenous β-cells are not clear. In the present study, we examined the changes of endogenous β-cell mass after islet transplantation in partially pancreatectomized mice.
Methods: Balb/c mice were 70% pancreatectomized, transplanted with syngeneic islets (group IV), and were
compared with pancreatectomized mice treated with insulin (group III) or no insulin (group II). Blood glucose levels and body weight were monitored. Remnant pancreas was obtained at 6 or 10 days after pancreatectomy, and mmunohistochemical staining was done for the evaluation of β-cell mass changes.
Results: Hyperglycemia and weight loss were induced after pancreatectomy. After islet transplantation or insulin treatment, blood glucose levels recovered to normal, and body weight started to increase. Plasma insulin levels were higher and β-cell mass was larger in group IV than in group II (P < 0.05). Especially, the difference of β-cell mass between them was more evident at 7 days as compared to at 3 day after transplantation. When compared to group III, group IV showed larger individual β-cell area after 7 days and larger β-cell mass after 3 days of islet transplantation (P < 0.05).
Conclusion: These observations indicate that islet transplantation plays a role in enhancing remnant β-cell
regeneration after partial pancreatectomy in rodents.
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KEYWORD
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Islet transplantation, Pancreatectomy, β-cell regeneration
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